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Dangal. Full Movie Download In Hindi. Subscribe to get instant updates on your Email for Dangal Full Movie Download in Hindi.Effect of lipopolysaccharide and interferon-gamma on the levels of cyclooxygenase-2, interleukin-6, and nitric oxide by cultured human osteoblastic cells.
Periimplant osteolysis is characterized by the influx of mononuclear cells and activation of the local immune response. Proinflammatory cytokines and prostanoids have been implicated as mediators of bone resorption in the periimplant tissue. The authors explored the effect of lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma), 2 cytokines involved in the local immune response, on the production of cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6) as well as nitric oxide (NO) by human osteoblastic cells (HOB) in culture. HOB were cultured with or without LPS (10 ng/mL) and IFN-gamma (1000 U/mL). Production of COX-2 protein and mRNA was assessed by Western and Northern blotting, respectively. IL-6 was assayed by ELISA in the supernatant. NO production was assessed by nitrite assay. COX-2 protein and mRNA were significantly increased by IFN-gamma as compared with control cells. LPS alone increased the production of NO. The addition of LPS and IFN-gamma in conjunction significantly decreased IL-6 production and increased COX-2 and NO production as compared with HOB cultures treated
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